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Articles from Genetic Engineering & Biotechnology News (GEN) which Highlights Recent Advances in Gene Expression and Function

Technology Focus is sponsored by Qiagen. This selection of recent articles from Genetic Engineering & Biotechnology News (GEN) highlights recent advances in Gene Expression and Function.

Obstacles to Exploitation of miRNA Falling


Next Step Is Better Tools to Achieve Full Potential of Analysis Techniques
By Catherine Shaffer

It's 2007, and at this point miRNA needs no introduction. Just in case you're late to the party, though, miRNAs are small, single-stranded nucleotides, which were originally believed to have little impact on gene expression and protein formation. In addition, miRNAs are accurate biomarkers for numerous diseases and are also involved in the expression of DNA. Scientists are still continuing to discover new roles for these nucleotides. How could we have possibly known that something so fascinating was to be found in the middle of absolutely nowhere? 

 Full Article

Whole Genome Amplification Strategies


Attention to Detail and Quality Control Are Essential
By Elizabeth Lipp

With drug pipelines needing a jump-start, many scientists are turning to long-forgotten samples in cold storage. At Cambridge Healthtech's recent "7th Annual G.O.T. Summit" in Boston, the spotlight was on whole genome amplification as presenters examined some of the challenges in genomic sample preparation. Topics covered included whole genome amplification and the impact of tissue fixation time on array comparative genomic hybridization (CGH) profiles; novel methods of genomic sample preparation; gene-expression profiling, and targeted nucleic acid sample-preparation methods for expression analysis.   

Full Article

Harnessing RNAi's Gene-Silencing Power


RNAi Is Trying to Come Into Its Own with Novel Delivery Systems and Profiling Techniques
By Kathy Liszewski

The emerging science of RNAi is tackling early hurdles and making strides to realize its potential for treating diseases ranging from cancer to HIV. New advances will be highlighted at Select Biosciences' "RNAi World Congress" conference later this month in Philadelphia. Current progress includes enhancing delivery by using reversibly charged liposomes; engineering small, organic compounds called nanotransporters; and monitoring individually transfected cells. Other innovations are being made to profile and quantify miRNA as well as to harness the power of endogenous RNAi machinery such as Dicer.     

Full Article


Glycoanalysis of Recombinant Proteins


Qproteome GlycoArray Kit for Determining the Pattern & Abundance of Glycosylation Epitopes
By Peter Porschewski, Ph.D., and Thibault Geoui, Ph.D.

Glycosylation is the most versatile and one of the most abundant of all co- and post-translational modifications. It results from the addition of sugar residues to a protein backbone to form a glycoprotein. Glycosylation plays an important role in many biological functions, including immune defense, fertilization, viral replication, parasitic infection, cell growth, inflammation, and cell-cell adhesion.   

Full Article


Novel Methodologies Allow Greater Accessibility to qPCR


By Nicholas D. Rose

Currently, real-time PCR is one of the most important techniques used in genomic analysis. The data it provides can be invaluable to determining disease mechanisms and discovering ways to target these diseases. Two new products, StepOne(tm) from Applied Biosystems (www.appliedbiosystems.com) and Qiagen's (www.qiagen.com) QuantiFast(tm), are making this technique more accessible to a wider range of researchers, according to officials at both firms.   

Full Article

About GEN

Genetic Engineering & Biotechnology News


is the most widely read publication in the biotechnology industry and the only high-frequency publication focused on international bioindustry news. Established in 1981, GEN is also the first biotechnology trade publication, having covered the industry since its inception.

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GEN is published by Mary Ann Liebert, Inc.
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